A new gene for flower color (crw, cream wings)
 in Pisum sativum L. located on LG I

Święcicki, W.K., Gawłowska, M. and Nawrot, Cz.                                                    Inst. of  Plant Genet.

                                                                                                           Polish Acad. of Sci., Poznań , Poland

       A line with tolerance to Aphanomyces euteiches was obtained in 1998 from Dr. Earl Gritton ( University of Wisconsin-Madison , USA ) as WI 9301.  Before its inclusion in the Polish Pisum Gene Bank in Wiatrovo (as accession Wt 11166) it was thoroughly characterized, and a new character, cream-colored wings on the flowers in the a genotypic background (no anthocyanin synthesis) was observed.  The sail and keel remained white.

      The line Wt 11166 was crossed to Wt 3527 with normal white color of sail, wing and keel. The F1 plants had white flowers. Among 128 F2 plants 96 were white flowering and 32 showed cream wings.

      The line Wt 11166 was crossed to testerlines with gene markers for different linkage groups. An inheritance of the new character was confirmed. Data in the Table 1 show clear monohybrid segregation with recessive nature of cream wings even though some populations are not particularly large. The reason is the A genotype of  some testerline parents resulting in  ¼ of total F2 population with the a-allele on which cream wings can be observed (see tables 1, 2 and 3). Gene symbol crw is suggested.

Table 1. Monohybrid segregation for the investigated gene cream wings and gene markers in the linkage group I in F2 populations of the linkage test cross Wt 11166 x : 11238 (K.2296), 11540 (K.2423), 11288 (K.2425), 15860 (K.2559) 11777 (K.2558), 11909 (K.2720), 10357 (K.2796), 11143 (K.2799) 10488 (K.2797), Id 860 (K.2800)

Cross

Gene

Dom

rec

Total

chi square

K.2296

crw

33

10

40

0.07

K.2423

crw

43

11

54

0.62

K.2425

crw

42

7

49

3.00

K.2559

crw

27

9

36

0.00

K.2558

crw

56

22

78

0.43

K.2720

crw

113

56

169

5.97

K.2796

crw

20

7

27

0.01

K.2799

crw

83

35

118

1.37

K.2797

crw

18

4

22

0.54

K.2800

crw

56

19

75

0.00

 

Am1

111

41

152

0.32

 

Aero

136

53

189

0.93

 

I

152

34

186

4.48

 

A

152

37

189

2.96

 

Table 2. Distribution of phenotypes in F2 populations (Wt 11166 x testerlines) and the linkage test for the gene crw

Testerline

Pair of

genes

DD

Dr

rD

rr

Total

Joint chi square

Cr-O(±SE)

(per cent)

Phase

Wt 11238

A- Crw

1

1

33

10

43

2.0

66.4

±13.3

C

 

K-Crw

31

10

1

1

41

1.0

65.1

±9.6

R

 

Wb-Crw

29

9

4

1

43

0.0

46.9

±11.8

R

 

Tl-Crw

28

9

5

1

43

0.0

43.3

±12.2

R

 

Cp-Crw

25

9

8

1

43

0.8

35.4

±13.2

R

 

Te-Crw

24

9

9

1

43

0.8

33.3

±13.4

R

 

Gp-Crw

25

9

8

1

43

0.8

35.4

±13.2

R

 

S-Crw

30

9

3

1

43

0.0

51.5

±11.2

R

Wt 11540

Crw-Gp

29

14

11

1

54

5.2

27.8

±12.4

R

 

Crw-Wb

35

8

9

2

54

0.0

49.6

±10.2

R

 

Crw-A

2

40

1

11

53

1.0

58.4

±11.2

C

 

Crw-Aat2

20

7

3

2

32

1.4

58.9

±11.9

R

 

Crw-Lap1

21

6

4

1

32

0.0

48.1

±13.5

R

 

Crw-Pgm1

18

9

5

1

32

1.4

37.2

±15.0

R

 

Crw-Pgm2

17

10

3

2

32

0.0

48.2

±13.0

C

 

Crw-Aldo

19

8

3

2

32

0.0

56.4

±12.3

R

 

Crw-Skdh

18

9

4

1

32

1.0

40.3

±14.6

R

 

Crw-Acp1

7

5

2

2

16

0.0

45.3

±17.6

C

 

Crw-Pgdp

18

8

5

1

31

1.4

38.8

±15.0

R

Wt 11288

Crw-A

1

42

1

7

49

2.0

73.6

±13.1

C

 

Crw-St

35

7

6

1

49

0.0

47.4

±11.0

R

Wt 15860

Crw-Creep

24

3

8

1

36

0.0

50.0

±12.5

R

 

Crw-A

1

26

1

9

35

2.0

64.7

±14.6

C

Wt 11777

Crw-Arg

44

12

17

5

78

0.0

48.9

±8.4

C

 

Crw-Art

45

11

18

4

78

0.0

48.6

±8.6

R

 

Crw-Wlo

44

12

19

3

78

0.4

42.3

±9.2

R

 

Crw-Pl

41

14

18

4

77

0.4

56.0

±9.1

C

Wt 11909

Crw-Def

87

25

40

13

165

0.2

51.7

±5.7

R

 

Crw-Chi5

80

17

44

9

150

0.0

49.4

±6.2

R

 

Crw-Wa

81

21

45

9

156

0.2

46.3

±6.2

R

 

Crw-Pgdp

80

32

41

14

167

0.1

47.7

±5.9

R

 

Crw-Skdh

90

20

40

12

162

0.7

54.2

±5.6

R

Wt 10357

Crw-Tac

16

4

6

1

27

0.0

44.3

±15.3

R

 

Crw-Pet

17

3

5

2

27

1.5

61.2

±12.5

R

Wt 11143

Crw-N

54

29

25

8

116

1.7

42.7

±7.5

R

 

Crw-Fa

71

12

26

8

117

1.2

58.3

±6.3

R

 

Crw-V

68

15

23

10

116

2.3

59.3

±6.2

R

Wt 10488

Crw-Fa

17

0

3

1

21

2.3

72.0

±11.9

R

 

Crw-Led

15

3

3

1

22

0.0

57.1

±14.7

R

 

Crw-N

13

3

3

1

20

0.0

55.1

±15.8

R

 

Table 3. Distribution of phenotypes in F2 population (Wt 11166. crw x Id 860. am1 aero I ; K.2800) and the linkage test for the gene crw

 

Phenotype

 

Gene pair

Phase

DD

Dr

rD

rr

Total

Joint chi square

Cr-O ±SE

(per cent)

Am1-Crw

R

1

0

28

9

38

1

34.3

14.1

Am1-Aero

C

100

11

12

29

152

55.4

16.0

3.3

Am1-I

R

82

27

40

0

149

11.5

18.6

7.8

Crw-Aero

R

22

34

15

4

75

10.2

26.8

10.6

Crw-I

C

54

1

13

6

74

12.5

15.2

4.6

Aero-I

R

100

34

52

0

186

17.4

16.2

7.1

       Linkage test showed that in most of F2 populations Wt 11166 (crw) x testerline deviations from a correct dihybrid segregation crw – gene marker were not found (Table 2). An exception was K. 2800 (Wt 11166 x Id 860) segregating for crw and A am1 aero I (Table 1). Id 860 is the IPG PAS selection with above mentioned markers of LG I. Dihybrid segregation was disturbed for all gene pairs (joint chi square from 10.2 to 55.4) except for Am1 – Crw. Data for this gene pair are “artificial” as am1 is hypostatic to a  and crw to A. Data in the Table 3 suggests the following locus order: Crw – I– Aero – Am1. This is in agreement with the current Pisum map (1) and provides LG I with a new morphological marker below i. A confirming linkage test should include the af marker.

 1.       Weeden, N.F., Ellis, T.H.N., Timmerman-Vaughan, G.M., Święcicki, W.K., Rozov, S.M. and Berdnikov, V.A.  1998.  Pisum Genetics 30: 1-4.