Four more symbiotic mutants obtained using EMS mutagenesis of line SGE

 Tsyganov, V.E.¹, Voroshilova, V.A.¹,               ¹All-Russia Res. Inst. for Agric., St. Petersburg, Russia

Rozov, S.M.², Borisov, A.Y.¹ and                       ²Inst. of Cytology and Genetics, Novosibirsk, Russia

Tikhonovich I.A.¹

      Previously we described isolation of series of symbiotic mutants (4) on laboratory line SGE (3). In this study four new symbiotic mutants: SGENod^--6, SGENod^--7, SGEFix^--3 and SGEFix^--4 were obtained using EMS mutagenesis (0.15% EMS during 10 h) of line SGE. Mutant SGENod^--6 is unable to form any nodules, mutant SGENod^--7 forms few or no nodules. Mutants SGEFix^--3 and SGEFix^--4 form pink-greenish non-fixing nodules. Tests for allelism have revealed that mutant SGENod^--6 is allelic to mutant E69 (sym7) (2), mutant SGEFix^--3 is allelic to mutant P63 (sym26) (1, and G. Duc, M. Sagan, pers. comm.). For mutants SGENod^--7 and SGEFix^--4 tests for allelism have not been completed yet.

 Acknowledgement: This work was supported by RFBR (98-04-49883).

1.    Duc, G. and Messager, A.  1989.  Plant Science 60: 207-213.

2.   Kneen, B.E. and LaRue, T.A.  1986.  Pisum Newslett. 18: 33.

3.   Kosterin, O.E. and Rozov, S.M.,  1993.  Pisum Genetics. 25: 27-31.