Four more symbiotic mutants obtained using 
EMS mutagenesis of line SGE

Tsyganov, V.E., Voroshilova, V.A.,             All-Russia Research Institute for Agricultural Microbiology,

Borisov, A.Y., Tikhonovich I.A. and          196608, St.-Petersburg, Pushkin 8, Podbelsky ch. 3, Russia

Rozov, S.M.                                      Institute of Cytology and Genetics, 630090, Novosibirsk, Russia

      Previously we described isolation of series of symbiotic mutants [1] on laboratory line SGE [2]. In this study four new symbiotic mutants: SGENod^--6, SGENod^--7, SGEFix^--3 and SGEFix^--4 were obtained using EMS mutagenesis (0.15% EMS during 10 h) of line SGE. Mutant SGENod^--6 is unable to form any nodules, mutant SGENod^--7 forms few or no nodules. Mutants SGEFix^--3 and SGEFix^--4 form pink-greenish non-fixing nodules. Tests for allelism have revealed that mutant SGENod^--6 is allelic to mutant E69 (sym7) [3], mutant SGEFix^--3 is allelic to mutant P63 (sym26) [4, and G. Duc, M. Sagan, pers. comm.]. For mutants SGENod^--7 and SGEFix^--4 tests for allelism have not been completed yet.

Acknowledgement: This work was supported by RFBR (98-04-49883).

1.      Tsyganov, V.E., Borisov, A.Y., Rozov, S.M., and Tikhonovich I.A. 1994. Pisum Genetics. 26: 36-37.

2.      Kosterin, O.E. and Rozov, S.M. 1993. Pisum Genetics. 25: 27-31.

3.      Kneen, B.E. and LaRue, T.A. 1986. PNL. 18: 33.

4.      Duc, G. and Messager, A. 1989. Plant Science 60: 207-213.