PNL Volume 21 1989 RESEARCH REPORTS                    75
A NEW GENE HETEROPHYLUS (het) ON CHROMOSOME 7
Swiecicki, W. K.                                                  Plant Breeding Station, Wiatrowo
62100 Wagrowiec, Poland
A mutant, with changed morphology of the stipules was induced after seed treatment of line Wt 3527 by a combined dose of 200 r NF + 0.014% NEU (1). The stipules are strongly reduced at the base of stem, narrowed and opened at middle order nodes and different in size at the top nodes (Fig. 1). The mutant line Wt 11291 was included in the Wiatrowo pea gene bank in 1981.
Locus identity tests were made with five other mutation cases of similar type and type lines for stipules reduced (Wt 10232, st), stipulae asymetricae (Wt 11072, stia) and stipula imminuata (Wt 11520, stim). The F1 results showed that the character described above in line Wt 11291 is controlled by a locus different from any of the other genes in the com­parison. For these reasons the name heterophylus and the symbol het is proposed.
In linkage studies, the F2 population of cross Wt 11291 (het) x Wt 11744 (tl wsp r) was analyzed in the greenhouse in 1988. Undisturbed monohybrid segregation was shown for het and tl with significant de­viations for wsp and r (Table 1A). The data in Table IB show significant deviations from independent assortment for gene pairs tl-r, het-r and het-tl but no deviations for gene pairs involving wsp. The recombination fractions (Table IB) suggest the following gene order:
1. Swiecicki, W.K. 1984. PNL 16:84-86.
Fig. 1. A new gene het.
7(,
PNL Volume 21            1989         RESEARCH REPORTS
Table 1. Phenotypic distribution in an F2 population from a cross between Wt 11291 (heterophylus) and Wt 11744 (testerline).
A. Monohybrid F2 segregation
Phenotype
Total
Chi-square (3:1)
Het
154
Tl 173
Wsp
180
R
187
het 61
tl 42
wsp
36
r 35
215 215 216 222
1.3
3.4
8.0
10.1
B. Joint segregation of Het with Tl, Wsp and R
Phenotype
Total
Joint
Chi-
square
Recomb. fract.
S. E.
Phase
Het Tl
Het tl
het Tl
het tl
115
39
58
3
215
11.8
25.4
6.3
R
Met R
Het r
het R
het r
121
33
60
1
215
13.7
16.9
6.6
R
Tl R
Tl r
tl R
tl r
169
4
12
30
215
113.9
7.9
1.9
C
Het Wsp 129
Het wsp 25
het Wsp 50
het wsp 11
215
0.2
51.8
5.0
R
Tl Wsp 141
Tl wsp 32
tl Wsp 38
tl wsp 4
215
1.9
60.7
5.7
C
Wsp R 149
Wsp r 31
wsp R 33
wsp r
3
216
2.2
61.6
5.7
C
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