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NEW GENES LINKED TO R AND
Tl IN PEA: HI STONE HI SLOW FRACTION AND SEED ALBUMIN K9
GENES
Smirnova, O.G., S. M. Rozov, and
V. A. Berdnikov
Institute of Cytology and
Genetics, Academy of Sciences of the USSR
Siberian Division, Novosibirsk,
USSR
Seed proteins are convenient
genetic markers. The use of traditional methods of salt extraction
followed by sodium dodecylsulphate electrophoresis gave an
opportunity to observe already well studied storage proteins vicilin and
legumin. Perchloric acid extraction and acetic acid-urea electrophoresis
(1) enabled us to study a new group of poorly examined proteins. The major
component of a perchloric extract is a protein named by us K9 (it contains
nine lysine residues). Protein
K9 represents 1% of the total pea seed proteins and has a molecular weight
of about 10,000.
Our studies show that K9 has two
molecular forms. A fast electropho-retic variant was found only in the
wild peas subspecies syriacum and elatius. To map the gene
coding for the K9 protein (gene Sa-K9) we crossed line WIR 2524
(ssp. elatius) with the tester-line NGB 1238. An analysis of
F2 progeny (n=115) has shown that the slow and fast K9
molecular variants (K9S and K9F) are coded by codominanl allelic genes.
The Sa-K9 gene is located 13.5 ± 2.6 map units from gene tl
and 16.5 ± 2.4 map units from gene
r.
It was reported earlier that the
gene encoding the pea HI histone slowest fraction (His-1) is
located near gene r (2). Using the Sa-K9 gene as an
additional marker, we investigated the location ol the His-1 gene
in relation to the r-tl segment of chromosome in the cross
'Sprint' (alleles R,
Tl, His-lS, Sa-K9F) and tester-line NGB 1018 (alleles _r, _tl, His-lT.
Sa-K9S). Electrophoretic patterns of K9 and His-1 proteins are shown in Fig. 1.
Segregation of analyzed genes in F2 plants is represented in
Table 1. Heterozygous phenotype Tl/tlw was determined as
flat tendrils, heterozygotes Rr were revealed from the seed F3
analysis. |
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1. Berdnikov, V.A., and F. L. Gorei. 1975.
Molekularnaja Biologija. 9:699-705 (in Russian).
2. Rozov, S. M., V. S. Bogdanova, and V. A.
Berdnikov. 1986. Genetika (USSR)
8:2159-2166. |
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Table 1. Phenotypic distribution of F'2 plants segregating for genes R_, JJ_,
His-I , and Sa~K9 from cross Sprint x NGB 1018
(n=98). |
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Fig. 1.
Acetic acid-urea electrophoretic patterns of pea seed perchloric extracted proteins and pea H1
histone.
A. Seed proteins, arrow indicates
K9
B. T3C fragments of HI
histone
a) WIR
2524; b) F1 WIR 2524 x NGB 1238; c) NGB 1238; d) Sprint; e) F1 Sprint x NGB 1018; f) NGB
1018 |
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