LINKAGE RELATIONS OF GENES af-i ON CHROMOSOME 1 AND curl-tl ON CHROMOSOME 7 IN PEAS (Pisum sativum L.)

PNL Volume 19 1987 RESEARCH REPORTS 77

LINKAGE RELATIONS OF GENES af-i ON CHROMOSOME 1 AND curl-tl ON
CHROMOSOME 7 IN PEAS (Pisum sativum L.)

Trifu, I. Research Institute for Cereals and Industrial Crops

Fundulea, Romania

The gene af which converts the leaflets into tendrils is
largely used in breeding programs for improving standing ability
of pea. Its location on chromosome 1, first reported by
Khangildin (2), subsequently has been confirmed by others
(1,3,4,5,6,9) but the values of linkage intensity between af and
i obtained by different authors has varied widely: 45.8% (2),
5.36% (3), 14.5% (9), 7.47% (1), 8.3% (4), 3.7% (5), 3.72% in the
greenhouse, and 5.69% in the field (6). The factors leading to
such great variation of recombination fraction values are still
unknown.

Genetic analysis of the F2 population resulting from the
cross F81-1261 (af af i i) x cv. 'Auralia' (Af Af I I) confirmed
the linkage af-i on chromosome 1. The recombination
fraction value was 7.20 + 1.27 (Table 1).

Table 1. Analysis of joint segregation in F_? for genes af and i.


Gene pair	XY	Xy	xY	xy	Chi-square			Recomb. fract.	S.E.
Gene pair	XY	Xy	xY	xy	X	Y	Linkage	Recomb. fract.	S.E.
Af i	314	16	16	450	0.67	0.67	328.8***	7.20	1.27

*** Significant at 0.001

The mutant curl was obtained by Sidorova and Uzhintzeva (8)
by treating 'Torsdag' seeds with EMS. The same mutant was ob-
tained also at I.C.C.P.T.-Fundulea by gamma irradiation of seeds
of line F70-413. The line possessing the mutant gene was de-
signated MF-13 curl. Marx (7) has located the gene curl on
chromosome 7.

Analysis of F2 populations of crosses of MF-13 curl, line
with several lines possessing marker genes for different chromo-
somes confirmed the location of curl gene on chromosome 7 (Table
2). The recombination fraction value between the genes curl and
tl was 26.95 + 3.79.

1. Gujov, Iu. L. 1975. Izv. Akad. Nauk USSR Ser. Biol. 2:219-

236.

2. Khangildin, V. V. 1966. Genetika (USSR) II(6):88-96.

3. Khangildin, V. H. and V. V. Khangildin. 1969. Tr. Bash-
kirskogo Nil selskogo hoz. 3:40-61.

4. Kielpiski, M. 1982. PNL 14:30.

5. Marx, G. A. 1983. PNL 15:43-45.

6. Marx, G. A. 1984. PNL 16:49-51.

7. Marx, G. A. 1986. PNL 18:45-48.

8. Sidorova, K. K. and L. P. Uzhintzeva. 1975. PNL 7:56.

9. Snoad, B. 1971. PNL 3:43.

PNL Volume 19

1987

RESEARCH REPORTS

Table 2. Analysis of joint segregation in F2 for curl and different
gene markers.


Gene pair	XY	Xy	xY	xy	Total	Chi-square			Recomb. fract.	S.E.
Gene pair	XY	Xy	xY	xy	Total	X	Y	Linkage	Recomb. fract.	S.E.
Curl Af	258	91	77	29	455	0.70	0.46	0.06 ns
Curl St	155	42	48	20	265	0.06	0.36	1.77 ns
Curl Le	196	61	80	19	356	1.50	1.21	0.98 ns
Curl Gp	99	39	36	12	186	0.06	0.58	0.19 ns
Curl Pl	164	50	54	20	288	0.07	0.07	0.40 ns
Curl -Tl	288	123-	108	8	527	2.51	0.01	30.60 **	26.95	3.79

** Significant at 0.01

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