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76 PNL Volume 19 1987 RESEARCH REPORTS
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CONFIRMING DATA FOR MAPPING ISOZYMIC LOCUS Aat-p
Swiecicki, W. K. Plant Breeding Station, Wiatrowo, Poland
and B. Wolko
An isozyme linkage map for Pisum was published by Weeden in
1985 (1), providing an additional group of markers useful for gene mapping, as well as for genotype/cultivar characterization (2). One of these enzymes was analyzed at Wiatrowo, i.e. Aspartate aminotransferase - Aat-p (=Glutamate oxaloacetate transaminase Got-1). The testerline WL 851 (allele B2, slow for Got-1) was crossed with Wt 3838, cv. 'Arabal' (allele B1, fast). These lines also have different alleles of genes A and Lf. Plants of the F1 generation showed the dominant phenotype for A and Lf as well as a heterozygotic pattern for Aat-p (Got-1). An undisturbed monohybrid segregation in F2 was observed for A, Lf , as well as for Aat-p (Table 1A). A 3:1 segregation for Lf-lf was obtained on the basis of considering individuals with the first flowering node below 6 as recessives. For Aat-p (Got-1) co-dominant type of inheritance was observed (1:2:1) but for linkage calculations allele B1 of Got-1 (fast variant) was added to the heterozygotes to get the 3:1 segregation pattern. The dihybrid segregation be- tween Aat-p (Got-1) with A and Lf produced the following CrO values (Table 1B). Aat-p---------------------27.0-------------A-------18.7--------Lf
I-----------------------------32.0-----------------------------I
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The above confirms Weeden's data showing linkage between
Aat-p and A as 30 units and also extends the linkage relations to the Lf locus of chromosome 1. |
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1. Weeden, N.F. 1985. The Pea Crop, P.D. Hebblethwaite,
M.C. Heath, and T.C.K. Dawkins, eds. Butterworths, London. pp. 55-66. 2. Wolko, B. and W.K. Swiecicki. 1987. PNL 19:89.
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Table 1.
Phenotypes distribution in an F2 population segregating for Aat-p (Got-1) from the cross Wt 851 x Wt 3838.
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A.
Monohybnd F1 segregation
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B. Joint segregation of Aat-p with A and Lf
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