ELECTROPHORETIC EVIDENCE OF A SPECIFIC SEED ALBUMIN OF PISUM FULVUM

66 PNL Volume 16 1984

RESEARCH REPORTS

ELECTROPHORETIC EVIDENCE^1/ OF A SPECIFIC SEED ALBUMIN OF PISUM FULVUM

Przybylska, J., E. Kozubek Institute of Plant Genetics

Polish Academy of Sciences, Poznan, Poland

Blixt, S. Weibullsholm Plant Breeding Institute

Landskrona, Sweden

In previous investigations of electrophoretic (EP) seed albumin

patterns in Pisum, nine such EP-patterns, designated I to IX, were

distinguished. The patterns were formed by different combinations of

several well defined protein bands, designated a - f. Albumins cor-

responding to the characteristic bands, called "specific albumins", were

recovered in the sephadex fraction with MW about 40,000 (the S2

fraction). Only in the case of the Pisum form representing EP III,

i.e., line WL-1490 called P.cinereum, was the characteristic band b not

observed on an electropherogram of the S2 fraction (1, 2, 3).

The recently reported side-by-side slab gel electroophoresis of the

S2 fractions, performed for the Pisum forms representing EP I-V and VII-

IX (Pisum forms with EP VI seem to have no S2 fraction) revealed a

variation in electrophoretic mobility of apparently homologous bands.

Thus, the b band of EP V from P. fulvum WL-1256 migrated faster than the

b band from P. sativum WL-110, representing the commonly occurring EP I.

The band b from line WL-1256 was then called b₁ and the b band from line

WL-110 was designated b₂ The band b from P. sativum VIR 1987 with

EP VII, a pattern found in a number of accessions originating from

Transcaucasia and obtained from VIR, corresponded to band b₂. These

observations raised the question whether or not band b₁ is characteris-

tic of P. fulvum.

In this work five accessions of P. fulvum and 152 accessions repre-

senting other Pisum forms were analyzed. Of these other forms 143

accessions represented P. sativum, five accessions P. elatius and four

accessions P. humile, having b-containing EP patterns EP I and EP VII.

— Cooperative investigation performed under an Agreement between the

Royal Swedish Academy of Sciences and the Polish Academy of Sciences.

The project involves cooperation with Prof. Dr. R. Kh. Makasheva from

N.I. Vavilov All-Union Research Institute of Plant Industry, VIR,

Leningrad, USSR (Agreement between the Ail-Union Academy of Agricultural

Sciences of USSR and the Polish Academy of Sciences) and Dr. Ch. Lehmann

from Zentrallnstltut fur Genetlk und Kulturpflanzenforschung of German

Academy of Sciences, Gatersleben, GDR. The study was carried out under

Project MR 11/7, coordinated by the Institute of Plant Physiology of the

Polish Academy of Sciences.

PNL Volume 16 1984

RESEARCH REPORTS

The material analyzed was obtained mainly from the Pisum Gene Bank at

Weibullsholm and from N. I. Vavilov All-Union Research Institute of

Plant Industry, VIR, Leningrad (2, 3). For every Pisum accession one

seed (the cotyledons) was analyzed. Crude protein extracts with large

proportions of albumins were subjected to electrophoresis on slab gels

as described in a previous paper (3).

The results indicated that of the Pisum accession examined only

Pisum fulvum accessions have the b₁ band while the other accessions have

band b₂. The b₁ band was observed in four P. fulvum accessions having

the EP V characteristics of the taxon and also in one P. fulvum acces-

sion having a deviating EP pattern ("Population 1" wild-growing in

Israel). Thus the b₁ band seems verily to be characteristic of

P. fulvum. Fig. 1 shows the electrophoretic patterns of the Pisum

fulvum accessions and of some accessions representing other taxa.

1. Jakubek, M. and J. Przybylska. 1979. Genetica Polonica 20:369-

380.

2. Przybylska, J., S. Blixt, J. Hurich, and Z. Zimniak-Przybylska.

1977. Genetica Polonica 18:27-38.

3. Przybylska, J., Z. Zimniak-Przybylska, E. Kozubek, and S. Blixt.

1984. Genetica Polonica (in press).

Fig. 1. Slab gel showing that the b band (b₁) in P_. fulvum accessions

(4-8) migrates faster than the b band (b₂) in other Pisum taxa (1-3,

9-10). The patterns shown were produced by the following accessions:

(1) - WL-110, P. sativum (EP 1); (2) - WL-226, P. elatius (EP I); (3) -

WL-1951, P. sativum (EP I); (4) - P. fulvum population wild-growing in

Israel, obtained from the Hebrew University of Jerusalem and called

"Population 1" (EP deviating); (5) - VIR 3397, P. fulvum (EP V); (6) -

Jl 224a, P. fulvum (EP V); (7) - Jl 224b, P. fulvum (EP V);

(8) - WL-1256, P. fulvum (EP V); (9) VIR 1987, P. sativum (EP VII);

(10) - VIR 2381, P. sativum (EP VII).