SDS-ELECTROPHORETIC INVESTIGATION ON SEED ALBUMINS IN PISUM

PNL Volume 16 1984

RESEARCH REPORTS

Grillo, S. and R. Rao Plant Breeding Institute

University of Naples, Portici, Italy

The e lectrophoretic analyses of seed albumins in P isum are of

interest in taxonomic identification (1). In this genus, however,

scanty data are available on variation of albumin subunit molecular

weights.

The seed albumins of three taxa were investigated by SDS-

electrophoresis; six lines of P. abyssinicum, seven of P. fulvum

obtained from John Innes Institute, and one of P. sativum (cultivar

'Proteo') were analyzed.

Albumin extracts were obtained from cotyledons of mature seeds (2)

and .submitted to SDS-polyacrilamide gel electrophoresis on slab gels

with 15% running and 4% staking, according to Laemmli (3).

Two major bands, ranging in the 28 Kd and 18 Kd zones, were always

present (Fig. la and lb). Slight differences were found among P. abys-

sinicum lines, mainly regarding bands falling in the 18 Kd zone. Larger

differences were found among P. fulvum lines, particularly for the bands

ng in the 15-20 Kd area; line JI 1010 (Fig. lb) showed a specific

pattern characterized by the presence of an additional band of molecular

weight around 23 Kd. The P. sativum patterns were less different from

the P. abyssinicum than from the P. fulvum profiles.

1. Przybylska, J., S. Blixt, J. Hurich, and Z. Zimniak-Przybylska.

1977. Genetica Polonica 18:27-38.

2. Grillo, S. and R. Rao. 1982. Ann. Fac. Agraria, University of

Naples Serie IV XVI:l-5.

3. Laemmli, U. K. 1970. Nature 227:680-685.



	PNL Volume 16 1984 RESEARCH REPORTS



	Fig. 1 - SDS-electrophoregram of seed Albumins in P. abyssinicum (a), in P. sativum (P) and in P. fulvum (b).