58
PNL Volume 15
1983
RESEARCH REPORTS
 
 
ISOZYME VARIATION AT SELECTED LOCI IN PISUM
Weeden, N. F.
NYS Agricultural Experiment Station, Geneva, NY, USA
Several recent studies have demonstrated the existence of allelic
variants (allozymes) at isozyme loci in Pisum and have used such varia-
tion to help elucidate relationships among taxa within the genus (1,2).
In an effort to identify additional allozymic variation for linkage and
biochemical studies I surveyed approximately 100 diverse lines and ten
USDA Plant Introduction accessions. The latter group included samples
labelled P. elatius. P. jomardii. and P. sativum ssp. syriacum. abys-
sinicum and hortense. The 43 isozyme stains used in the survey
permitted the visualization of products from 77 distinct loci. Of
these, 40 were found to exhibit allozymic forms. The list of currently
available isozyme variants is given in Table 1 along with the lines in
which the rarer variants were discovered. This author would appreciate
information regarding polymorphism at additional isozyme loci not in-
cluded in Table 1.
1. Parzysz, H. , J. Przybylaaka and S. Blixt. 1982. PNL 14:55-56.
2. Przybylska, J., H. Parzysz and S. Blixt. 1981. PNL 1 3:42-43.
PNL Volume 15
1983
RESEARCH REPORTS
59
Table 1. Polymorphic loci identified in Pisum
Locus
Acid pnosphatase-1
Acid pnosphatase-2
Acid phosphatase-3
Aldolase-1
Alcohol dehydrogenase-1
Aspartate aminotransf erase-2
Aspartate aminotransferase-3
Amylase-1
Diaphorase-2
Esterase-1
Esterase-2
Esterase-3
Esterase-4
Fructose bisphosphatase-1
Fructose bisphosphatase-2
Fumarase-1
Galactosidase-1
Galactosidase-2
Glucose 6-P dehydrogenase-2
Glutamate-pyruvate transaminase-1
Isocitrate dehydrogenase-1
Leucine aminopeptidase-1
Leucine aminopeptida se-2
Malate dehydrogenase-1
Malate dehydrogenase-2
Malate dehydrogenase-3
Malic enzyme-1
Mannose phosphate isomerase
Peroxidase-2
Phe-pro peptidase
Phosphoenolpyruvate carboxylase-1
Phosphoenolpyruvate carboxylase-2
Phosphoglucomutase-1
Phosphoglucomutase-2
Phosphoglucose isomerase-2
6-Phosphogluconate dehydrogenase-1
6-Phosphogluconate dehydrogenase-2
3-Phosphoglycerate kinase-1
3-Phosphoglycerate kinase-2
Shikimic dehydrogenase-1
Line possessing rare variant*
GAM A1 078-236
GAM A1078-23*
GAM A1078-236
P.I.3*3993
(see reference 2)
many
P.I. 2 42 0 27; GAM A1078-234
P.I. 343997
P.I. 268480
GAM B77-257
GAM B77-257
GAM A78-237
many
P.I. 3 5 86 1 5
GAM C282-243
many (GAM C282-243; C282-295
P.I. 268480
P.I. 268480
P.I. 358612
P.I. 343993
GAM A78-237
many
P.I. 273209
P.I. 343997
P.I. 343997
P.I. 242027
P.I. 3 5 86 1 2
P.I. 343993
GAM C282-172
many (P.I. 343985)
P.I. 343972
P.I. 343972
GAM B77-260
many
P.I. 343972
many
many
P.I. 358612
P.I. 358612
many
"GAM" refers to lines obtained from Dr. G.A. Marx; "P.I." refers to
accessions obtained from the Northeast Regional Plant Introduction
Station, Geneva, NY; "many" refers to cases in which each allele was
found in many different lines.