POLYMERIC GENES FOR WAXLESSNESS

8 RESEARCH REPORTS

PNL Volume 13

1981

Gottschalk, W.

Institute of Genetics, University of Bonn, Federal Republic of Germany

The following eleven waxless mutants have been selected after irradiatingj

seeds of the initial line:

A. Fully fertile genotypes (Fig. 1):

ll6 - Weakest degree of waxlessness. Small amounts of wax on the lower side

of the leaflets and on the top side of the stipules.

109B - Weak degree of waxlessness especially clearly visible at the stipules.

Intermediate between 116 and 423.

423, 445A - All green plant organs including the pods completely waxless.

Late ripening. Both mutants phenotypically identical.

B. Genotypes with strongly reduced fertility or sterility:

142A - Waxless flower mutant. Plants smaller than the control material.

Petals and filaments remaining within the calyx giving a bud-like appearance

to the fully developed flowers. Style jutting out of the calyx thus preventing

self-pollination. The plants are sterile although their gametes are fully

functional.

417B - Small and weak plants with degenerating areas at the margins of leaflet!

and stipules in the lower part of the plants. Reduced wax content in the

seedling stage; nearly normal during flowering stage.

250A - Small, waxless plants; very weakly fertile.

129 - Plants small; completely waxless. Fertility so low that the mutant

can only be maintained in heterozygous condition.

112A - Plants small, weakly waxless like 116, small greenish flowers, late.

Maintenance only through heterozygous plants.

C. Lethal genotypes:

99E, 413 - Waxless lethal mutants, dying after having formed 4 foliage leaves.1

The mutant genes of Group A can be regarded as polymeric genes influencing

the formation of wax each in a different way. Their genetic relations have

been studied by means of hybridization as follows:

F1's from 109B x 445A had normal wax and the F_? segregated as follows:

Obs. 24 normal : 7 like 109B : 8 like 445A

Exp. (9:7) 21.9 : 17.1

F 's from 116 x 445A had normal wax and the F₂ produced:

Obs. 27 normal : 11 like 116 : 8 like 445A

Exp. (9:7) 25.9 : 20.1

F1 's from 423 x 445A were waxless. The F2, generation likewise was uniformly

waxless. I

The following conclusions can be drawn from these findings: The genes

of mutants 109B and 116 are not allelic to the gene of mutant 445A. They

are polymeric and presumably not linked. The genes of 423 and 445A are either

identical or allelic since they cause the same kind of waxlessness (see below).

Crosses between mutants L09B and 116 have not yet been made, so we do not

yet know whether these two genes are polymeric or allelic.

Theoretically, waxlessness would be a disadvantageous character, especially

in dry summers because of increased transpiration. This, however, was not

apparent for the 4 mutants just mentioned (Fig. 1). Their mean values for

PNL Volume 13 1981

RESEARCH REPORTS

the character "number of seeds per plant", although somewhat lower than the

control values of the initial line, were equal to or higher than other fertile

mutants of our collection.

The question whether the genes of the phenotypically identical mutants

423 and 445A are identical or allelic has been studied in two recombinants:

- Recombinant R 836 derived from the cross of mutants 445A x 46C (early

flowering).

- Recombinant R 837 derived from the cross of mutants 423 x 46C.

The two recombinant types were, as expected, phenotypically identical.

They are homozygous for both mutant genes, causing complete waxlessness and

formation of flowers at very low nodes of the plants. Their seed production

was somewhat lower than that of the waxless mutants (Fig. 1). In the phyto-

tron, however, certain differences between the two genotypes were noted with

regard to flower formation. Under short-day conditions (12 h darkness), the

plants of both the recombinants aborted the first few floral buds. Later,

however, normal, fertile flowers were produced. Thus, the recombinants are

genetically early flowering, although the environment alters the genotypic

expression. This anomaly was much more strongly expressed in recombinant

R 837 (=423/efr) than in recombinant R 836 (=445A/efr); i.e. the plants of

R 837 were even later than those of R 836 although they are identical with

regard to the gene efr for earliness (Fig. 2). This difference could be an

indication that the two genes for waxlessness of mutants 423 and 445A are

not identical but allelic.

Fig. 1. Seed production of 4 waxless

mutants and 2 waxless recom-

binants as related to the control

values of the initial line=100%.

Each dot gives the mean value for

one generation.

Fig. 2. The number of days to

flowering for plants of each

of 4 genotypes grown under

short-day (12 h) conditions

in the phytotron. Each dot=

1 plant.