LOCATION OF NEW TRANSLOCATION IN PISUM

RESEARCH REPORTS

PNL Volume 12

1980

Ezhova, T. A. and S. A. Gostimski

Moscow State University, USSR

A study of 6-ray-induced chlorophyll mutant lines produced from the

'Capital' variety (normal structural type of chromosomes) revealed a recipro-

cal translocation in one of the lines (chlorina-mutant line No. 4). Karyo-

type analysis indicated participation in the interchange of chromosome 3

and one of the metacentric chromosomes (1 or 2) (Fig. 1). Studies of F1

hybrids produced by crossing line No. 4 x line 21 T(l-7) and line No. 4 x

line 83 T(3-5) showed the presence of hexavalents and four bivalents in the

cells of the first meiotic metaphase (Fig. 2). This confirmed the partici-

pation of chromosomes 1 and 3 in the interchange.

The translocation T(l-3) found in line No. 4 and a chlorina-mutation

from this same line, tentatively localized in chromosome 7, are apparently

non-connected mutational events induced simultaneously in initial cells of

the M1 seed following 6-irradiation.

Homozygous translocation T(l-3) was selected out in a pure line. The

plants of the T(l-3) line were not dissimilar phenotypically from the plants

of the initial variety.

Fig. 1. Karyotype of plants from

line No. 4 with homozygous

translocation.

Fig. 2. First meiotic metaphase

in the F1 hybrids

(line 4 x line 21 T(l-7).