Pisum Genetics   

Volume 25

1993

Research Reports

pages 60-63

Flowering in pea: a mutation from Lfd to lfa and a summary of induced Lf mutations

Taylor, S.A. and

Murfet, I.C.

Department of Plant Science, University of  Tasmania

Hobart , Tasmania 7001, Australia

Four alleles Lfd > Lf > lf > lfa have been identified at the Lf (late flowering) locus which shows close linkage with the basic gene for anthocyanin production, A (5, 7, 21). These four alleles result in minimum flowering nodes of 15, 11, 8 and 5, respectively, counting from the first scale leaf as node 1 (10). The actual flowering node observed depends on the background for the other flowering genes and the environmental conditions used (10, 14). As a guide, the values in Table 1 give the flowering node ranges usually observed in summer field conditions at Novosibirsk, Russia, and under an 18 h photoperiod in the glasshouse at Hobart, Tasmania, for plants with the background E Sn Dne Ppd. This background is found in many domestic cultivars (14). The genotype at the Hr locus has little effect on the flowering node under an 18 h photoperiod (6). However, under mild (> 17°C) temperatures and 8 h short day conditions, plants with genotype lf e Sn Dne Ppd Hr may produce in excess of 50 vegetative nodes (6). Thus the Lf alleles do not determine the maximum flowering node.

The Lf gene is about ten times as susceptible to mutation as any of the other major flowering genes in pea (14) and 21 induced mutations have now been identified at this locus (Table 2). The very early flowering mutant XVIII/17 was induced in cv. Vesna by 10 krad of gamma radiation from a 60Co source (M. Vassileva, pers. comm.). On the basis of allelism tests, Uzhintseva and Sidorova (18) concluded that line XVIII/17 was an lf  a mutant. However, they did not report the genotype of the initial line, Vesna.

We have now obtained results (Table 3) which indicate that Vesna carries the Lfd allele. Under an 18 h long day photoperiod (see Table 1 for details), Vesna tended to flower 2-4 nodes later than the standard late (L-type; 10) line, L24. Line 24 carries the same Lf allele as the Lf type line, L65E, and it has white flowers (a). In the F2, there was a strong association between white flowers and late flowering, and coloured flowers and very late flowering (Table 3), as would be expected if Vesna carried the Lfd allele in coupling phase with A. There were two obvious recombinants out of 60 F2 plants which leads to a recombination fraction of 3.3 ± 2.4%. This value is slightly less than the usual value of around 8-10%. The proposed Lfd/- segregants overlap the Lf/Lf segregants at nodes 18 and 19 but the Lf alleles are known to display incomplete dominance (1, 5, 7, 11) and Lfd/Lf heterozygotes may well account for this overlap. Under the 18 h photoperiod, Vesna tended to flower about two nodes earlier than the type line, WL1771 (= Hobart line 16), and the Lf/Lf segregants in the F2 tended to flower 2 to 3 nodes earlier than the Lf parent, L24. These results can be explained if Vesna carries a polygenic background which modifies the expression of the Lf alleles toward earliness. Mutant XVIII/17 flowered at node 7 (Table 3) which is consistent with genotype lfa (7) as concluded by Uzhintseva and Sidorova (18).

Table 1. Usual flowering node range for plants homozygous for alleles Lfd, Lf, lf or lfa grown under summer field conditions at Novosibirsk, Russia (18) or in the glasshouse at Hobart, Tasmania, under an 18 h photoperiod (natural daylight extended before dawn and after dusk by light from a 1:1 mixture of 40 W white fluorescent tubes and 100 W incandescent globes providing 25 mmol m-2 s-1 at pot top). The temperature in the glasshouse is usually maintained above 13°C. Node counts start from the first scale leaf as node 1 and the flowering gene background is taken as E Sn Dne Ppd and Hr or hr.

Conditions

Genotype

Lfd/Lfd

Lf/Lf

lf/lf

lfa/lfa

Novosibirsk: summer field

20-22

13-16

10-11

7-9

Hobart : glasshouse 18 h

20-24

13-19

9-13

6-8

Table 2. A summary of mutations identified at the Lf locus.

Mutation type

Initial line

Mutant line

Mutagenic agent

Author mutant

Allelism tests

Lfd -> Lf

'Dominant'(WL1771)

WL1770

Neutrons

20

8

Lfd -> lf

"

WL1769

Neutrons

20

8

Lfd -> lfa

Vesna

XVIII/17

10 krad gamma

19

16, 18

Lf -> lf

Torsdag

K319

NEU

17

9, 18

 

"

K320

NEU

17

18

 

"

K320/1

NEU

17

18

 

"

K326

NEU

17

18

 

Falensky 42

K398

EMS

17

18

 

Porta

Wt11790

200 r Nf

15

11

 

"

Wt11791

200 r Nf

15

11

 

Paloma

Wt1795b

NEU

15

13

 

Ranny Zeleny

R9

Callus

2

12

 

Ramonsky 77

I/178

100 r Nf

19

1

Lf -> lfa

Dippes Gelbe Viktoria

46c

X rays

3

8

 

Parvus

P745d

DES

4

8

 

"

L629

NMU

17

18

 

Torsdag

K2

Gamma

17

9, 18

 

"

K578

EMS

17

18

 

Falensky 42

K400

EMS

17

18

 

Saratovsky mestniy

K418

EMS

17

18

 

Kaliski

Wt11796

500 r Nf

15

13

a DES = diethyl sulphate, EMS = ethyl methane sulphonate, NEU = nitroso ethyl urea, Nf = fast neutrons, and NMU = nitroso methyl urea.

b This mutant allele falls between lf and lfa (13).

Table 3. Node of flower initiation data for the standard Lfd line (WL1771), early flowering mutant XVIII/17 (ex Vesna), cv. Vesna (A), the standard late (L-type) line L24 (Lf a), and F1 and F2 populations from the cross Vesna x L24. The plants in the last five rows were grown simultaneously. All plants were grown in the glasshouse under an 18 h photoperiod. Node counts started from the first scale leaf as node 1. The brackets indicate the suggested F2 segregation for Lf/Lf and Lfd/- plants.

Line or Cross

Node of flower initiation

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

WL1771

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1

7

6

5

XVIII/17

3

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Vesna

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2

1

 

 

L24

 

 

 

 

 

 

 

 

 

 

2

2

 

 

 

 

 

 

F1

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1

2

 

 

Vesna

 

 

 

 

 

 

 

 

 

 

 

 

 

1

3

 

 

 

L24

 

 

 

 

 

 

 

 

 

 

 

1

3

 

 

 

 

 

F1

 

 

 

 

 

 

 

 

 

 

 

 

 

2

 

 

 

 

F2 (a/a)

 

 

 

 

 

 

[1

1

2

5

3

2

1]

 

[1]

 

 

 

F2 (A/-)

 

 

 

 

 

 

[1]

 

 

 

 

[3

13

14

8

5]

 

 

 The data in Table 2 now include examples of forward mutation at the Lf locus of the type Lfd to Lf, Lfd to lf, Lf to lf, Lf to lfa, and the most extreme case Lfd to lfa. No examples of back mutation have yet been observed. The agents used to induce these mutations include physical and chemical mutagens, and in one case (2) the mutation arose in callus culture. With a score of 1 for each step down in the allelic series Lfd, Lf, lf, lfa, physical agents achieved a mean score of 1.67 ± 0.24 (n = 9) and chemical agents a mean of 1.45 ±0.16 (n= 11). These two means are not significantly different (P > 0.3) and bearing in mind that the three treatments to initial lines with the top allele Lfd are all with physical agents, there is no evidence that radiation has caused any more severe change than chemical agents. However, the two cases of gamma radiation have both resulted in large changes from Lfd to lfa and Lf to lfa.

  1. Arumingytas, E.L. and Murfet, I.C. 1992. Pisum Genetics 24:32-36.

  2. Ezhova,T.A., Bagrova, A.M. and Gostimski, S.A. 1990. PNL 22:15-17.

  3. Gottschalk, W. 1960. Züchter 30:32-34.

  4. Monti, L.M. and Scarascia-Mugnozza, G.T. 1967. Genetica Agraria 21:301-312.

  5. Murfet, I.C. 1971. Heredity 27:93-110.

  6. Murfet, I.C. 1973. Heredity 31:157-164.

  7. Murfet, I.C. 1975. Heredity 35:85-98.

  8. Murfet, I.C. 1978. PNL 10:48-52.

  9. Murfet, I.C. 1982. In Documentation of Genetic Resources: A Model, Eds
    S. Blixt and J.T. Williams, IBPGR, Rome, pp. 45-51.

  10.  Murfet, I.C. 1985. In Handbook of Flowering, Vol. IV, Ed. A.H. Halevy, CRC
    Press, Boca Raton, Florida, pp. 97-126.

  11. Murfet, I.C. 1991. Pisum Genetics 23:16-18.

  12. Murfet, I.C. and Ezhova, T.A. 1991. Pisum Genetics 23:19-25.

  13. Murfet, I.C. and Groom, K. 1984. PNL 16:57-58.

  14. Murfet, I.C. and Reid, J.B. 1993. In Peas - Genetics, Molecular Biology and
    Biotechnology, Eds D.R. Davies and R. Casey, CAB International, Wallingford,
    U.K. pp. 165-216.

  15.  Swiecicki, W.K. Plant Breeding Institute, Wiatrowo, Poland.

  16. Taylor, S.A. and Murfet, I.C. 1993. Pisum Genetics 25:60-63.

  17. Uzhintseva, L.P. and Sidorova, K.K. 1979. Genetika 15:1076-1082.

  18. Uzhintseva, L.P. and Sidorova, K.K. 1988. PNL 20:39-40.

  19. Vassileva, M. Institute of Genetics and Plant Breeding, Sofia, Bulgaria,

  20. Wellensiek, S.J. 1969. Z. Pflanzenphysiol. 60:388-402.

  21. White, O.E. 1917. Proc. Amer. Phil. Soc. 56:487-589.