Pisum Genetics 

Volume 24


Research Reports

page 16

A second gene for leaf edge necrosis, len-2, located on chromosome 5

Apisitwanich, S.


Swiecicki, W.K.


Wolko, B.


Department of Genetics, Kasetsart University

Bangkok, Thailand

Inst. of Plant Genetics, Polish Academy of Sciences

Poznan, Poland

Plant Experiment Station, Wiatrowo

Wagrowiec 62100, Poland

Several mutants characterised by papery necrosis of the leaf tissue were isolated by Swiecicki (3). The degree and type of necrosis varied but the phenotypes were generally similar to those conferred by len (1) and bulf (2). The mutant line Wt11319, isolated from cv. Kaliski after treatment with 350 r Nf + 0.014% NEU, has a phenotype very similar to len which causes necrosis of the leaf edge margins (1). The leaflets and stipules of the new mutant have a necrotic, papery margin with a variable degree of expression. The only difference between the two mutants is that len causes dotted necrosis of the leaf edge whereas our mutant shows whole region necrosis of the margin.

To test for allelism, mutant Wt11319 was crossed with the type lines for len (Wt15861) and bulf (Wt15872). In both crosses the Fl had a normal phenotype indicating that mutant Wt11319 is not allelic with len or bulf. The new mutant showed monogenic recessive inheritance. We therefore propose a new locus, len-2 (leaf edge necrosis), with Wt11319 as the type line for the len-2 allele.

To test for linkage, line Wt11319 was crossed with marker lines Wt11288, Wt11143, Wt11745 and Wt15860. No evidence of linkage was found for len-2 and markers b, st and M on chromosome 3, v, fa and n on chromosome 4, and tl, coch and r on chromosome 5. However, evidence of disturbed dihybrid segregation was found for len-2 and markers located in the lower half of chromosome 5 according to the recent linkage map of Weeden et al (4). Data from the F2 of cross Wt11319 (len-2 Creep) x Wt15860 (Len-2 creep) indicated a distance of 20 9 cM between len-2 and creep (57 Len-2 Creep, 24 Len-2 creep, 26 len-2 Creep and 0 len-2 creep; c2 = 9.9, P < 0.01) and data from the F2 of cross Wt11319 (len-2 Gp) x Wt11238 (Len-2 gp) indicated a distance of 20 8 cM between len-2 and gp (87 Len-2 Gp, 43 Len-2 gp, 22 len-2 Gp, 0 len-2 gp; c2 = 10.1, P < 0.01). We believe that len-2 may be located between gp and creep but further tests are necessary to determine the position of the locus more precisely.

  1. Marx,G.A. 1980. Pisum Newsl. 12:52-53.

  2. Sharma, B. 1973. Pisum Newsl. 5:46.

  3. Swiecicki, W.K. 1984. Pisum Newsl. 16:84-86.

  4. Weeden, N.F., Ambrose, M. and Swiecicki, W.K. 1991. Pisum Genetics 23 Cover.